221 
HPLC- Gradient Program: 
Runtime [min] 
Flow rate [pL/min] 
A [%] 
B [%] 
-15 
170 
85 
15 
0.1 
170 
85 
15 
4.0 
170 
70 
30 
16.0 
220 
50 
50 
25.0 
220 
30 
70 
39.0 
220 
5 
95 
48 
220 
5 
95 
Eluent A: 
Eluent B: 
Oven: 
MS system: 
Water, 10 mM ammonium acetate, 10 mM acetic acid 
Methanol, 10 mM ammonium acetate, 10 mM acetic acid 
23 °C 
API 2000 (Applied Biosystems), ESI source, negative 
mode, MRM 
Table 83 summarises the MS parameters for the target compounds and their internal 
standard (ISTD) labelled with 13 C. 
Table 83: Parameters of the target substances: molecular weight (MW), retention time (RT; LC-MS 
conditions see above) and masses used for analysis in SIM mode (m/z) 
Substance 
MW [g/mol] 
RT [min] 
Quantifier [[m/z] 
Qualifier [m/z] 
Pentachlorophenol 
264 
33.6 
264.8 
266.8 
13 C-Pentachlorophenol 
270 
33.5 
270.8 
272.8 
TCPy 
197 
23.9 
195.9 
197.9 
Std. 100ng/ml 
I 
TCPi 
' 
PCP 
5 10* 15 20 
*25 
*30 
35 4Ô 45 
Time, min 
Figure 52: HPLC-MS Chromatogram (SIM) of a standard solution of TCPy and PCP