178 
Biota samples 
Relative and absolute recoveries of the analytes in fish liver show a wider range and 
larger variation (see Table 73 and Table 74) than the two other matrices. This is caused 
by interfering effects of matrix during the analysis and problems observed with the GPC 
clean-up column. The absolute recovery of Di4-Trifluralin is low because separation 
from the lipids via GPC is difficult and, therefore, a large amount of trifluralin is lost 
during separation. Quantification nevertheless is reliable because the relative recovery 
of trifluralin - referred to Di4-Trifluralin - is good (Table 73), and both have a 
chemically identical behaviour. 
Table 73: Relative recoveries [%] of the analytes in biota samples * 
Analyte 
Lima lim 
spike: 
3.2 pg/kg 
lipid 
Lima lim 
spike: 
211 pg/kg 
lipid 
Gadu mor 
spike: 
44 pg/kg lipid 
Gadu mor 
spike: 
3 pg/kg lipid 
Plat fie 
spike: 
0.8 pg/kg 
lipid 
Chlorpyrifos- 
ethyl 
143 
105 
109 
117 
132 
Chlorpyrifos- 
methyl 
87 
114 
80 
68 
80 
Endosulfan I 
105 
90 
61 
111 
104 
Endosulfan II 
147 
(500) 
57 
145 
127 
Trifluralin 
129 
106 
111 
110 
100 
* Lima lim: Limanda limanda, dab; Gadii mor: Gadua morhua, cod 
Piat fle: Platichtys flestis, flo under 
Table 74: Absolute recoveries [%] of the ISTD referred to D 6 -Chlorpyrifos-methyl as recovery standard 
in biota samples; addition of the recovery standard (0.004 ng/pL) after extraction and clean-up. 
Analyte 
Recovery [%] Lima lim 
(2 experiments) 
(M±STD) 
Recovery [%] 
Gadu mor (one 
experiment) 
Recovery [%] Plat fie 
(one experiment) 
D io-Chlorpyrifos-ethyl 
79±3 
58 
20 
D 4 -Endosulfan I 
65±5 
68 
27 
D 14 -Triflur aliti 
20±22 
60 
54