141 
with a 025 pm film of DB5-MS (5% phenyl-95 %-methylsiloxane, J&W Scientific, 
Folsom, USA). The injected volume was 1.5 pi in the splitless mode (2 min splitless 
time). The injector temperature was 240 °C. He was used as carrier gas (99,999 % 
purity, Carbagas, Switzerland) at a head pressure of 68,9 kPa (10 psi). The following 
temperature programme was employed: 90 °C isothermal for 2 min, with 30 °C/min to 
150 °C, then 4 °C/min to 250 °C, 2 min isothermal. 
Also here an HP 5989B mass spectrometer was applied in the ECNI mode (Hewlett 
Packard, Palo Alto, USA). The temperature of the transfer line was 250 °C, of the ion 
source 200 °C and of the quadrupole 100 °C. Methane was used as reagent gas 
(99,995 %, Carbagas, Switzerland) at a pressure of 1-1.6 hPa (0.9-1.1 Torr). 
Table 45 summarises the selected masses for chlordanes in the selected ion monitoring 
mode. The most abundant chlorine isotope signal was selected for quantification and the 
second one for confirmation. The metabolites oxy-chlordane, trans- and c/.s-hcpta- 
chlorepoxide could not be completely separated on the selected capillary. c/.s-Hcpta- 
chlorepoxide and oxy-chlordane co-eluted in front of /ran.s-hcptachlorcpoxidc. Quan 
tification of cis-heptachlorepoxide was therefore carried out with the not disturbed but 
less abundant mass m/z 388 and of oxy-chlordane with m/z 424. The presence of the 
chlordanes MC5 and MC7 was confirmed by their retention relative to c/.s-chlordanc 
(MC5: 0,982; MC7: 1,021, (Karlsson, 1999; Karlsson etal., 2000)).