132 
5.6.2 Reference standards for chlordane 
The reference compounds listed in Table 41 were used for method development, 
validation and quantification of chlordanes. 
Table 41: Manufacturer and concentrations chlordane solutions used for quantification. The purity was 
>99 % except for MC8 and 4,5-dichlorchlordene. 
Compound 
Manufacturer 
Concentration 
frans-Chlordane 
Promochem 
8.08 ng/pl* 
r/.v-Chlordane 
Promochem 
4.98 ng/pl* 
frans-Nonachlor 
Promochem 
43.18 ng/pl* 
r/.v-\onachlor 
Promochem 
44.32 ng/pl* 
rz'.v-Heptachlorepoxide 
Ehrenstorfer 
10 ng/pl* 
rra/z.v-Heptachlorepoxide 
Ehrenstorfer 
10 ng/pl* 
Heptachlor 
Promochem 
188.4 ng/pl* 
Oxychlordane 
Ehrenstorfer 
10 ng/pl* 
13 Ci 0 -fra«.s-Chlordane 
Cambridge Isotope Laboratories 
100 ng/pl** 
MC8 
Karls son (2000) 
1 ng/ pi* 
4,5 -Dichlorochlorden 
29 
1 ng/ pi* 
Octachloronaphthalene 
Ehrenstorfer 
10 ng/pl* 
T etrachloronaphthalene 
Ehrenstorfer 
10 ng/pl* 
in cyclohexane, in n-nonane 
5.6.3 Clean-up procedure for fish (PCAs and chlordanes) 
Up to 7 g of the pooled fish liver samples were mixed with the tenfold quantity of 
water-free sodium sulphate (dried at 600 °C overnight) in a mortar. The free-flowing 
powder was transferred to a glass column (length 30 cm, ID 2.0 cm). 10 ng of the 
internal standards 1 ’C io-/ran.s-ch lordanc dissolved in 10 pi of cyclohexane were added 
to the top of the column. Elution was carried out by drop wise addition of 250 ml of n- 
hexane/dichloromethane (1+1, v/v). The eluate was completely evaporated to dryness 
with a Turbo Yap (Zymark, Hopkinton, USA).