69
Table 14: PCA quantification of different sPCA mixtures (51-69 % Cl) by ECNI with total response
factors corrected for the chlorine content. The chlorine contents calculated from ECNI-LRMS
measurements are indicated in brackets. The expected amount, the measured amount and the relative error
are given. Deviations between expected and found chlorine content are caused by the presence of
additives which are not detectable by ECNI.
sPCA mixture
[% chlorine content]
Expected
amount [ng]
Measured
amount [ng]
Relative
deviation [% ]
Standards used for establishment of linear function (R 2 =
0.9730, y = 0.00041x - 0.02391)
51 (58.7 %C1)
1500
1553
4
55 (60.2 % Cl)
1500
1572
5
59 (61.7 %C1)
1605
1602
0
64 (64.5 % Cl)
1070
1030
4
69 (66.1 % Cl)
1605
1724
7
Control standards
53 (59.8 % Cl)
1500
1379
8
59 (62.7 % Cl)
1500
1690
13
63 (63.6 % Cl)
1500
2030
35
Hordalub 80 (56 % Cl
1500
1902
27
Table 15: Comparison of sPCA determination in fish liver by ECNI according to Tomy et al. (1997)
using three commercial references of different chlorine content (51 %, 55 % and 63 %) and via the total
response factor approach. Calculated chlorine contents are given in brackets.
Sample no.
Quantification according to Tomy et al. [ng/g ww]
Total response
factor approach 3
Standard 51
Standard 55
Standard 63
(58.7 % Cl)
(60.3 % Cl)
(64.0 %C1)
1 (61.9 %C1)
794
138
43
73
2 (62.9 % Cl)
1055
184
58
82
Standard 51
Standard 55
Standard 63
(58.2 % Cl)
(59.9 % Cl)
(63.4 %C1)
3 (62.2 % Cl)
209
94
21
29
4(61.7 %C1)
358
161
37
57
Standard 51
Standard 55
Standard 63
(58.6 % Cl)
(60.2 % Cl)
(63.5 %C1)
5 (61.1 %C1)
168
81
17
34
6 (62.0 % Cl)
158
76
16
24
7 (60.6 % Cl)
184
88
18
47
8 (61.8 %C1)
2494
1197
250
408
9 (59.2 % Cl)
733
352
73
521
10 (59.9 % Cl)
56
27
6
21
“Chlorine content correlation determined with three SCCP mixtures (51 %, 55.5 % and 63 %
Cl, R 2 = 0.999 for sample 1 and 2, R 2 = 0.969 for sample 3 and 4, R 2 = 0.964 for sample 5 to
10)
